Year: 2015 | Month: April | Volume 5 | Issue 1
Elucidation of Molecular Basis of Neutrophil Apoptosis during Staphylococcal Mastitis in Crossbred Cows
Abstract:
Neutrophil apoptosis is a dynamic process following their recruitment to the site of infection that varies depending upon the type of challenge. The proposed study was designed to elucidate the role of classical mediators of apoptosis in neutrophils isolated from milk samples of crossbred Karan Fries cows suffering from subclinical (SCM) and clinical mastitis (CM). Milk samples were collected from 12 KF cows suffering from clinical mastitis caused by Staphylococcal aureus. Clinical mastitis was confirmed on the basis of CMT scoring, bacteriological evaluation, gross and morphological changes in milk and by counting milk somatic cells (SCC). Milk Poly Morpho-Nuclear Cells (PMNs) were isolated and apoptosis was studied. Neutrophil apoptosis was evaluated by studying the exteriorization of phosphatidyl serine, mitochondrial transmembrane potential, Caspase 3, 7, 8 and 9 by fluorescent microscopy. Results showed that apoptosis in neutrophils were mediated through exteriorization of membrane phosphatidyl serine; increased mitochondrial transmemebrane potential and activation of caspases 3, 7, 8 and 9 like other somatic cells. From the study, it was evident that neutrophils undergo induced apoptosis during Staphylococcal mastitis. The findings of the study provide an insight into the molecular basis of neutrophil apoptosis and form a basis to enhance the host immunity by the process of apoptosis modulation to combat the infections caused by the pathogen. The study provided a base for future studies by which neutrophil apoptosis can be modulated so as to enhance the phagocytic clearance of the microbes from the site of infection.
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